Acidosis Impacts on Southern Appalachian Brook Trout
The range of the Southern Appalachian Brook Trout (SABT) is greater than just the Great Smoky Mountains National Park (GSMNP) (see Figure 1), but GSMNP has been a pioneer in research related to brook trout genetics, population monitoring, restoration, and other brook trout studies by local, state, and federal agencies and a myriad of universities.
The SABT has experienced severe declines within its range for a number of reasons. One of the most significant, at least in the GSMNP, has been the episodic acidification of surface waters. Many studies have been conducted over the last decade looking at the issue and its impact on the physiology and survivability of the SABT. Environmental stressors such as headwater water chemistry changes, predominantly pH changes, have been shown to be the major source of headwater declines in brook trout range in GSMNP (Wesner et al. 2011). What this excerpt will focus on is the physiological mechanisms used by SABT to cope with acidosis in their environment.
GSMNP receives elevated levels of atmospheric sulfur and nitrogen deposition due to the burning of fossil fuels, automobile emissions and agricultural practices. When these nitrogen and sulfur compounds are combined with air and water, they create acids that contribute to the acidification of the soil and surface waters. Higher elevation streams are known to be more sensitive to acidic conditions because of their low acid neutralizing capability, resulting in higher rates of episodic stream acidification in the higher reaches of the park (>3500 ft) (Deyton et al. 2009, Neff et al. 2013).
Increased industrial emissions were caused by the burning of fossil fuels that release SO2, NO, NO2, and NH3 into the atmosphere, where it can attach water vapor and fall with precipitation in the form of H2SO4, HNO3, and NH4+ (Driscoll et al. 2003). GSMNP receives elevated rates of atmospheric acid deposition in comparison with other areas on the east coast, resulting in increased episodic stream acidification events, adding to the acidification of soil and surface waters in the park. Episodic stream acidification occurs when increased rates of atmospheric acid deposition occur, bringing increased precipitation to soils and water bodies, resulting in periods of increased stream flow and decreased water pH. Areas with heavy year-round rates of atmospheric acid deposition tend to have decreased soil pH. By decreasing the number of base cations (or positive ions like calcium, potassium and sodium) in the local geology, this limits buffering capabilities, and exacerbates the pH problem. Organic acids also contribute to the acidification of streams in the form of natural plant nitrates carried from surface soils into stream channels.
Acids leach into poorly buffered streams of the GSMNP through wet deposition (acid rain), dry deposition (pollutants that stick to leaves during dry periods, but are flushed from these and other surfaces during rain events), and naturally occurring organic acids in soils. When these acids run off into the watershed, they cause rapid declines in stream pH (Driscoll et al. 2003, Neff et al. 2009). Acidification events create a rapid change in stream pH and a general imbalance in the environmental ions available for ion exchange. The mechanism of acid stress in fishes is generally recognized as an ion regulatory disturbance that can lead to circulatory collapse and in extreme cases death.
Environmental acidifications are very taxing events on normal fish physiology. Fish are highly sensitive to even minute changes in environmental pH, which can result in changes in enzyme function. (Claiborne et al. 2002) Acid/base regulation is a greater challenge for fish than it is for terrestrial organisms primarily because of the use of water as a respiratory medium in contrast to air. As a result fish must ventilate their gills at a high rate in order to meet normal oxygen needs. The smallest of changes in environmental pH may result in relatively large changes in blood pH for a fish. To counteract any abrupt changes in internal pH, fish rely on the direct transfer of acid/base relevant ions (H+, HCO3-, NH4+, OH-) across primarily gill epithelial surfaces, but also the kidney and intestine have been shown to be involved (Evans, et al. 2005).
A variety of mechanisms have been postulated to be involved in acid/base relevant ion exchange. In freshwater fish, the epithelial mechanism associated with the excretion of excess acid has been shown to be the proton ATPase (H+-ATPase). (Perry et al. 2003, Evans et al. 2005)
This ATPase pathway is heavily ATP dependent, therefore it represents a high metabolic cost for any organism in which it is active. This pathway is not thought to be the only transporter associated with pH regulation, but is thought to significantly effect acid excretion in freshwater fish. When studying membrane transport proteins it quickly becomes apparent that this function is rarely a process of maintaining a single ion's homeostasis. These proteins work as a unit to maintain a balance between the organism and external environment.
The current range of the SABT has been created by a variety of historic and current geologic, geographic, and anthropogenic influences as well as recent environmental factors on the species, such as habitat loss and degradation, competition with nonnative fishes, climate change, and atmospheric acid deposition (Galbreath et al. 2001, Aunins 2015). Some declines of SABT populations can be originally attributed to deforestation of their native habitats by timber companies, the lack of forest decreased stream quality due to sediments and runoff flowing into the streams with the absence of a riparian buffer to control it (Karas 1997). The lack of shade that the recently logged timbers creates also allowed water temperatures to rise, forcing the fish to move to more shaded, cooler zones or die (Aunins 2015). The logging also impacted the riparian buffer which then impacts the chemistry of the streams.
The water quality of some streams and rivers, which SABT inhabit inside of the park, regularly fall outside of the standards set by the "Clean Water Act" putting these fish at risk of acidosis (GSMNP Water Quality Annual Report 2011). In fact in 2008, 12 Tennessee streams were listed as "Impaired" on the state 303d list due to low stream pH (mean pH values less than 6.0). In many areas of the park with SABT populations, there are high elevation, small basin watersheds, which typically have lower stream sodium content, low acid neutralizing capacity (ANC) of the stream and soil, and high water flow in storm events (Neff et al. 2013). Within these high elevation watersheds of GSMNP, it is believed that episodic acidification of streams associated with atmospheric acid deposition is the greatest threat contributing to the loss of SABT.
This difference in water chemistry around the park can create difficulties when completing water quality assessments and their impacts on the SABT populations, especially since there are adaptive differences within GSMNP SABT populations let alone across their entire range based on environmental adaptations and habitat differences. In other studies unique stream specific adaptations have been noted in SABT populations from one stream to another within the park. Though no study has been found to support the hypothesis it would be logical to assume there would also be differences across SABT range as well. Given this data and assumption it could be concluded that separate SABT populations would react differently to short and long term pH change events, therefore making a definitive comparison and conclusion difficult without more study.
The regulation of pH is not the only challenge for freshwater fishes;because of the low sodium content of freshwater environments, fish must maintain a hyperosmotic state compared to the external environment. Maintaining a specific hyperosmotic ion concentration prevents the fish from becoming too salt-depleted or overhydrated (Evans et al. 2005, 2008). This results in a hyperosmotic state in freshwater fishes in which the animal is constantly loosing salts (NaCl) to their external environment and gaining water. Because of the excessive water gain in low pH environments, fish pee a lot. In the chemical sense, ion balance and pH regulation are intrinsically linked. Research has shown the coupling of Cl- with HCO3- and OH- also Na+ with NH4+ and H+, provides passive channels for these molecules to regulate through. The internal osmolarity of freshwater adapted fish is much greater than that of the environment. This causes the fish to passively absorb water while simultaneously losing Na+ and Cl- ions to the environment. This causes that fish to produce copious amounts of hypotonic urine as well as actively up-taking Na+ and Cl- through gill epithelia as well as through ingestion.
The SABT is constantly undergoing changes in its internal pH due to atmospheric acid deposition affecting the acid balance of its native streams. These constant changes make it very difficult for these animals to maintain a systemic pH homeostasis or balance, although it is necessary for their survival. The aim of this research is to provide physiological information from SABT that can assist GSMNP in determining toxicity thresholds of the various acid deposition compounds. These data can be used to set air quality standards to guide emission reductions that will restore and maintain viable, healthy SABT populations within the park. To provide these data, the GSMNP fisheries department focuses a portion of their fish and water quality monitoring efforts on streams known to be low in sodium content in order to assess fish and stream health. These data will guide regulators and park managers in developing strategic GSMNP plans that will reduce acidification of park streams and provide the habitat conditions necessary to protect and preserve SABT populations within and across its borders for future generations.
Aunins, A.W., Petty, J.T., King, T.L., Schilz, M., and Mazik, P.M. 2014. River mainstem thermal regimes influence population structuring within an Appalachian brook trout population. Conserv. Genet. 16: 15–29
Claiborne, J.B., Edwards, S.L., and Morrison-Shetalr, A.I. 2002. Acid-Base regulation in fishes: Cellular and molecular mechanisms. Journal of Experimental Zoology 293 (3): 302-319
Deyton, E.B., Schwartz, J.S., Robinson, R.B., Neff, K.J., Moore, S.E. and Kulp, M.A. 2009. Characterizing Episodic Stream Acidity During Stormflows in the Great Smoky Mountains National Park. Water, Air, and Soil Pollution `96 (1-4): 3-18
Driscoll, C.T., Driscoll, K.M., Mitchell, M.J., and Raynal, D.J. 2003. Effects of acidic deposition of forest and aquatic ecosysyems in New York State. Environmental Pollution 123 (3): 327-33
Evans, D.H., Piermarini, P.M., and Choe, K.P. 2005. The multifunctional fish gill: Dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiological Reviews 85 (1): 97-177
Evans, D.H. 2008. Teleost fish osmoregulation : what have we learned since August Krogh, Homer Smith, and Ancel Keys. Am. J. Physiol. Regul. Integr. Comp. Physiol. 295: 704–713
Galbreath, P.F., Adams, N.D., Guffey, S.Z., Moore, C.J., and West, J.L. 2001. Persistence of native southern Appalachian brook trout populations in the Pigeon River system, North Carolina. North Am. J. Fish. Manag. 21: 927–934.
Karas, N. 1997. Brook Trout. The Lyons Press, Guilford, Connecticut.
Mikeworth, B. 2012. Identification and Localization of H+-ATPase. NHE2 and NHE3 in the Gills of the Southern Appalachian Brook Trout, Salvelinus fontinalis, Department of Biology, Appalachian State University
Moore, B.C. 2014. NPS Fisheries Research Reports 11-19-14 and 12-12-14. Fisheries Department, Great Smoky Mountain National Park Mount, D., Ingersoll, C., Gulley D., Fernandez, J., LaPoint, T., Bergman, H., 1988. Effect of Long-Term Exposure to Acid, Aluminum, and Low Calcium on Adult Brook Trout (Salvelinus fontinalis). 1. Survival, Growth, Fecundity, and Progeny Survival. Canadian Journal of Fisheries and Aquatic Sciences 45 (9): 1623-1632
Neff, K,J., Schwartz, J.S., Henry, T.B., Robinson, R.B., Moore, S.E., and Kulp, M.A. 2009. Physiological Stress in Native Southern Brook Trout During Episodic Stream Acidification in the Great Smoky Mountains National Park. Archives of Environmental Contamination and Toxicology 57 (2): 94-103
Neff, K,J., Schwartz, J.S., Moore, S.E., and Kulp, M.A. 2013. Influence of basin characteristics on baseflow and stormflow chemistry in the Great Smoky Mountains National Park, USA. Hydrological Processes 27 (14): 2061—2074
Perry, S.F., Shahsavarani, A., Georgalis, T., Bayaa, M. Furimsky, M., and Thomas, S.L.Y. 2003. Channels, pumps, and exchangers in the gill and kidney of freshwater fishes: Their role in ionic and acid-base regulation. Journal of Experimental Zoology Part A –Comparative Experimental Biology 300A (1): 53-62.
Schwartz, J., Meijun, C., Neff, K.J., Rolison, C., and Pobst, T. 2011. Great Smoky Mountains National Park 2011 Water Quality Annual Report.
Tem, W., Payson, P. 1986. Effects of Chronic Exposure of Sublethal pH on Growth, Egg Production, and Ovulation in Brook Trout, Salvelinus fontinalis. Canadian Journal of Fisheries and Aquatic Sciences 43: 275-280
Wesner, J.S., Cornelison, J.W., Dankmeyer, C.D., Galbreath, P.F., and Martin, T.H. 2011. Growth, pH Tolerance, Survival, and Diet of Introduced Northern-Strain and Native Southern-Strain Appalachian Brook Trout. Transactions of the American Fisheries Society 140 (1): 37-44