Canyonlands is famous for its mountain biking terrain, particularly for the 100-mile White Rim Road at the Island in the Sky. The Maze also offers some multi-day trip possibilities, though the logistics and roads are more difficult (for the support vehicles, not the bikes). Many of the roads in the Needles travel up wash bottoms and are unsuitable for bikes due to deep sand and water.

Permits are required for all overnight trips in the backcountry. Permits are not required for day rides. During the spring and fall, demand for permits frequently exceeds the number available. If you plan to visit Canyonlands during peak season, especially to camp along the White Rim Road, it is recommended that you make reservations well in advance. These permits can be reserved starting the second Monday in July for the next calendar year.

Mountain bikes groups must remain on established roads and camp in designated sites. There is no single-track riding in the park. A support vehicle is recommended for all multi-day bike trips as there are no water sources along most of the roads. Guided trips are available for many destinations within Canyonlands.