The owners or managers of these historic sites and interpretive facilities are certified partners with the National Park Service on the Trail of Tears National Historic Trail.
- Cherokee County Historical Museum
- in the two-story, stone Carnegie Library Building, 87 Peachtree Street, in Murphy.
- Junaluska Memorial and Museum
- at 1 Junaluska Drive in Robbinsville.
- Road from Ross to Ridge's
- a three-mile-long road that ran from Major Ridge's farm to John Ross's farm, both of which are now within the city of Rome. It is now a walk/bike path as part of the Downtown Heritage Trails.
- David Crockett State Park Trail Segment
- is at the entrance to David Crockett State Park, north of U.S. Highway 64 (also state highways 15 and 242), located approximately one mile west of downtown Lawrenceburg (Lawrence County). The street address is 1400 West Gaines Street
- Giles County Trail of Tears Interpretive Center
- 220 Stadium Street, Pulaski
- The Hermitage
- Andrew Jackson's longtime home, is at 4580 Rachels Lane, Hermitage. The site is located 12 miles east of downtown Nashville.
- James Brown Cherokee Plantation
- at 9521 Ooltewah-Georgetown Road, northeast of Ooltewah, Hamilton County.
- Nashville 1823 Toll Bridge Abutments
- adjacent to the Victory Memorial bridge over the Cumberland River, Nashville
- Port Royal State Park
- at 3300 Old Clarksville Highway, Adams. The park, located in eastern Montgomery County, is just north of State Highway 76; it is eight miles southwest of Adams and 15 miles east of Clarksville.
- Sequoyah Birthplace Museum
- at 576 State Highway 360, which is approximately one mile east of Vonore.
- Tennessee River Museum
- at 495 Main Street, Savannah. The museum is at the corner of Main Street (U.S. 64) and Adams Street, just a few blocks east of the Tennessee River Bridge.
- Wilson-Erwin House
- Tuscumbia Landing
- at the confluence of the Tennessee River (Pickwick Lake) and Spring Creek, near the foot of Blackwell Road, west of downtown Sheffield.
- Waterloo Landing
- at the foot of Main Street, Waterloo, Lauderdale County.
- Willstown Mission Cemetery
- near the corner of Godfrey Avenue and 38th Street NE, Fort Payne.
- State Road – Hill Cemetery, North Segment, segment of the State Road (CA-254) running northwest from Hill Cemetery
- 2.5 miles southeast of Fredonia on Hill Cemetery Rd. (Caldwell County)
- Berry's Ferry and John Berry's Homesite
- on the south bank of the Ohio River near the intersection of state highways 133 and 137, Livingston County.
- Big Spring
- Washington Street
- near Hawthorne Street, Princeton, Caldwell County.
- Crider Tavern Complex
- 90 Old Mexico Road, Fredonia.
- Gray's Inn (Stagecoach Inn)
- at 88 Graysville Road (State Highway 294), in Tiny Town, three miles west of Guthrie, Todd County.
- Radford Farm
- at 610 Dixie Beeline Highway (US 41), in Todd County between Pembroke and Trenton.
- Henry E. Davis Homestead
- 101 Hwy. 8, Steelville (Crawford County)
- Silas Brickey Schoolhouse and Spring Campsite
- on Westover Rd. (703) 0.5 miles east of the intersection with Cherry Valley Rd., 2 miles west of Huzzah (Crawford County)
- Arcadia Valley Campground
- State Highway 21 near the intersection of Old Highway 21, Pilot Knob, Iron County.
- Greene County Trail Segments
- former railroad right-of-way near the intersection of S. Golden Avenue and W. Republic Road, Springfield vicinity.
- Laughlin Park
- is near Roubidoux Spring, which is adjacent to Superior Road and just south of State Highway 17 (Historic U.S. Highway 66) on the eastern edge of Waynesville.
- Maramec Spring Park-Massey Iron Works
- at 21880 Maramec Spring Drive, just north of State Highway 8. The park is in eastern Phelps County, eight miles southeast of St. James.
- Snelson-Brinker Cabin
- on the north side of State Highway 8 in western Crawford County, eight miles west of Steelville and 10 miles from Exit 195 on Interstate 44.
- Star City Ranch Trail Segment
- is near State Highway U, eight miles north-northeast of Cassville.
- Stone County Historical and Genealogical Society Museum
- Museum of Native American History
- 202 SW O St., Bentonville
- Shiloh Museum of Ozark History
- 118 W Johnson Ave., Springdale
- Bell's Route Segment, Village Creek State Park
- in Cross and St. Francis counties, northeast of Forrest City. The Bell's Route segment, part of the old Memphis to Little Rock military road, is located approximately one mile south of the park's visitor center.
- Delta Cultural Center
- at 141 Cherry Street and 95 Missouri Street, both in downtown Helena.
- Fitzgerald Station and Farmstead
- at 2327 Old Wire Road near Springdale.
- Lake Dardanelle State Park
- at 100 State Park Drive in Russellville, Pope County. The park is located at 2428 Marina Road, which is four miles west of Russellville, off State Highway 326.
- La Petite Roche
- in the vicinity of Junction Bridge and at the foot of Rock Street, Little Rock
- Mount Nebo State Park
- at 16728 West State Highway 155, four miles west of Dardanelle.
- North Little Rock Riverfront Park
- on the north bank of the Arkansas River between Main Street Bridge and Interstate 30 Bridge, North Little Rock.
- Petit Jean State Park
- at 1285 Petit Jean Mountain Road, Morrilton.
- Pinnacle Mountain State Park
- at 11901 Pinnacle Valley Road, Little Rock. (The park is 15 miles west of Little Rock.)
- Webbers Falls Historical Society Museum
- 301 Commercial St. (corner of Commercial and Main), Webbers Falls
- Cherokee National Museum
- is three miles south of Tahlequah in the old Cherokee community of Park Hill. The Cherokee National Museum is a building in the center of the Cherokee Heritage Center complex.
- Fort Gibson
- at 907 North Garrison, on Oklahoma Highway 80 at the north edge of Ft. Gibson.
- Hunter's Home
- at 19479 East Murrell Home Road, in Park Hill, one mile southeast of the junction of State highways 62 and 82, and four miles southwest of Tahlequah.
- Camp Ground Cemetery
- adjacent to the Camp Ground Cumberland Presbyterian Church, at 50 Tunnel Lane, six miles east of Anna, Union County.
- Jentel Farm Trail Segment
- southwest of the intersection of Lick Creek Road and Carter Lane, 3 miles east of Anna
- McGinnis Cemetery Trail Segment
- State Highway 146 at Burns Road, six miles east of Anna.
- Toler Farm Trail Segment
- north of State Highway 146 near Burns Road, six miles east of Anna.
- Wagner Farm Trail Segment
- along Deputy Tower Road (Trail of Tears Road), five miles west of Golconda.
- Wayside Store and Bridges Tavern Site
- at 6980 State Highway 146 West, east of Pleasant Grove, Johnson County.