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Conserve O Gram 3/12: Identifying Mouse and Rat Damage in Museum Collections

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Rodents, such as mice and rats, are common vertebrate pests that endanger human health and cause extensive damage to museum collections. This Conserve O Gram (COG) provides information on identifying mouse and rat activity and damage.

See NPS Museum Handbook, Part I (MH I), Chapter 5: Biological Infestations for information on Integrated Pest Management (IPM), pest identification, inspection, monitoring, trapping, and rodent control action plans. See also COG 3/11 Identifying Museum Insect Pest Damage and COG 2/8 Hantavirus Disease Health and Safety Update.

Mouse and Rat Biology

Mice and rats can readily climb, jump, burrow, and gnaw with front incisor teeth. Mice can fit through spaces as small as 1/4-inch diameter and can jump one foot high. Rats are larger than mice but can fit through holes as small as 1/2-inch diameter and jump three feet high. They can chew through materials such as plaster, wood, concrete, and aluminum. The teeth grow continuously throughout the mouse or rat’s lifetime and are sharpened by gnawing. These rodents are fertile and capable of breeding large litters throughout the year. Mice and rats are associated with human food, clutter, and trash, and are often found in spaces housing museum collections.

How to Detect Mice and Rat Activity

Mouse and rat activity is often first discovered through sightings or hearing movement in walls and ceilings. Other evidence includes fecal matter (droppings), nests, urine stains, rub marks, hair, holes, chew marks, and the presence of concentrations of food and insect debris.

Work with an IPM Coordinator to conduct a safe rodent inspection. Wear gloves and a mask and use a flashlight, ultra-violet (UV) flashlight (urine fluoresces under UV) (Figure 1), and a camera during inspection. In cases of extensive rodent activity, additional Personal Protective Equipment (PPE) requiring a fit test and medical examination may be needed.

Mouse urine fluorescing under blacklight.
Figure 1: Mouse urine fluorescing blue-white on the bottom shelf of a cabinet, revealed using a UV flashlight. Many substances fluoresce, so UV flashlights help confirm when there are other signs of mouse activity in the area, as well. (NPS Photo)

Where to Look

Rodents prefer warmth, protection, quiet places to hide, and proximity to food and water. Look in secluded corners and other areas that provide harborage, including:

  • Collection storage areas

  • Collection preparation areas, such as box-making stations

  • Equipment and supply storage areas

  • Offices, including desk drawers and storage closets

  • Food preparation areas such as kitchens and break rooms

  • Garbage collection areas, especially if left overnight in buildings

  • Water sources, including dripping air conditioning units and leaking pipes

  • Structural features such as wall voids, electrical wiring holes, and false ceilings and floors

Evidence of Mouse and Rat Activity

Droppings

Mouse excrement is smooth with a characteristic ovoid shape with at least one pointed end. Although color can vary based on what the animal was eating, droppings are usually dark and similar in size (1/8 to 1/4 inch) and appearance to small dark grains of rice (Figure 2). Rodent fecal matter can stain surfaces (Figure 3). A single house mouse can produce 50-75 fecal pellets per day. Rat droppings are slightly larger (1/2 to 3/4 inch), wider, and blunt at the ends. Do not confuse mice and rat droppings with those of the American Cockroach, which are also blunt ended but have ridges and are smaller.

Mouse droppings on a wood surface
Figure 2: Mouse droppings. (NPS Photo)
Mouse fecal stains on a cement floor.
Figure 3: Mouse fecal stains on basement perimeter. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)

Urine Stains

Rodents urinate while walking or when perched on an edge, so urine trails can accumulate across a room. Accumulation of urine can stain soft and absorbent materials such as textiles and ceiling tiles made of mineral fibers. Urine splatters also darken and disrupt surfaces (Figures 4 and 5). Stained ceiling tiles located away from water sources present likely evidence of mouse or rat activity, rather than water damage (Figure 6).

Mouse urine stains on a book.
Figure 4: Mouse urine splatters on The Odyssey of Homer. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)
Splatters of mouse urine on a gray floor.
Figure 5: Mouse or rat urine splatters on corner floor. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)
Evidence of mouse urine in a ceiling tile.
Figure 6: Dark staining from mouse urine on drop ceiling tiles (photo courtesy of Thomas Parker, PhD, MuseumPests.Net)

Rub Marks

Because of their keen sense of smell and poor eyesight, mice and rats mark their territory and routes of navigation (“rodent highways”) with secreted oils and urine. Highways are visible as smear marks on walls and through-holes, and around pipes and on electrical wiring (Figure 67). As mice and rats travel, they may stain floors with feces and shed hair. These areas can smell musky from the concentration of bodily fluids.

Dark brown stains from mice on a white wire.
Figure 7: Dark rub marks (circled in red) on white electrical wire from mice using the wire to travel around the museum. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)

Nests

Mouse and rat nests are made from soft materials such as shredded paper, textiles, insulation, and other materials that are chewed and collected (Figure 78). They may be seen or smelled due to high concentration of urine and excrement.

Rolled papers that have been damaged by a mouse.
Figure 8: Paper ripped from architectural drawings form a mouse nest in the rear of a drawer. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)
A mouse sitting in a nest in a drawer
Figure 9: Mouse caught in a nest in a desk drawer in a historic house. Note the padding debris around it. (NPS Photo)

Feeding Stations

Mice and rats prefer feeding in protected places. These “feeding stations” can be identified by the presence of food crumbs, density of droppings and urine, and remains of eaten cockroach and other insect carcasses (Figure 810).

Debris from a mouse nest in a stage coach
Figure 10: Mouse nest in a stagecoach. (NPS Photo)

Damage to Objects

Mice and rats cause physical damage to a variety of museum objects, including fiber, leather, paper, textiles, and wood, as well as structural features. Examine the loss on an object for chew marks and irregular edges, as well as associated debris (Figures 11-15).

Rat teeth marks on a saddle
Figure 11: Chew marks on the edges of a leather saddle. (NPS Photo)
Hole from a mouse in a woven chair seat.
Figure 12: Hole in the woven seat of a chair from a mouse gnawing. Note irregular edges from incisors. (NPS Photo)
Blue and white striped blanket with holes from a mouse.
Figure 13: Damage to a woolen blanket from rat or mouse gnawing and seeking nest materials, harborage. (NPS Photo)
A paper document showing damage from a mouse.
Figure 14: Mouse gnawing leaves irregular edges on paper. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)
NPS Flat Hat with brim edges chewed by a mouse.
Figure 15: Rough edge on a ranger hat chewed by a mouse or rat. (NPS Photo)

Contribution to Larger Infestations

The presence of rats and mice attracts other pests. Dead mouse or rat bodies, nests, and remains can host invertebrate pests, such as clothes moths and dermestid beetles that feed on their hair and carcasses (Figure 146). Remains can result in increased invertebrate activity in other areas of a building.

Two small mice stuck to a glue pest trap.
Figure 16: Decomposing mouse carcass caught in insect sticky trap, surrounded by dermestid larvae casings. (Photo courtesy of Thomas Parker, PhD, MuseumPests.Net)

Exclusion, Monitoring, Trapping, and Housekeeping

Store collections in dedicated collections storage spaces within a well-sealed building envelope. Implement good housekeeping practices and regular and frequent inspection of all spaces housing collections.If mouse or rat activity is suspected and/or identified, perform exclusion techniques on the building and surrounding environment to prevent access. Implement a humane rodent snap trapping program to eliminate those already in the structure. Routinely monitor traps so changes in activity are detected quickly.

Selected References

Eckstein, Amanda and Joan Bacharach. “Hantavirus Disease Health and Safety Update.” Conserve O Gram 2/8. Washington, D.C.: National Park Service, 2014.

Klein, Denise. “Identifying Museum Insect Pest Damage.” Conserve O Gram 3/11. Washington, DC. National Park Service. 2008.

Museum Pests Working Group (MP-WG) <https://museumpests.net/>

National Park Service. Museum Handbook, Part I. Chapter 5: Biological Infestations. Washington, DC.

________ Chapter13: Museum Housekeeping


Fran Ritchie, Organic Objects Conservator
National Park Service
Harpers Ferry Center

The Conserve O Gram series is published as a reference on collections management and curatorial issues. Mention of a product, a manufacturer, or a supplier by name in this publication does not constitute an endorsement of that product or supplier by the National Park Service. Sources named are not all inclusive. It is suggested that readers also seek alternative product and vendor information in order to assess the full range of available supplies and equipment.

The series is distributed to all NPS units and is available to non-NPS institutions and interested individuals by subscription through the Superintendent of Documents, U.S. Government Printing Office, Washington, DC 20402; FAX (202) 512-2250. For further information and guidance concerning any of the topics or procedures addressed in the series, NPS Museum Management Program, 1849 C Street NW (NC 230), Washington, DC 20240; (202) 343-8142.

Last updated: January 23, 2026