Chapter 4
Kennewick Man Ancient DNA Analysis: Final Report Submitted to the Department of the Interior, National Park Service
By D. Andrew Merriwether and Graciela S. Cabana

Appendix 2
Photographic Log

Photo 1: Sample storage. Samples are stored in DNase/RNase free Sarstedt tubes.

Photo 2: Post decontamination. Kennewick MC3 sample has been scrubbed with a 10% bleach solution and UV-irradiated on all sides. Sample is ready for drilling.

Photo 3: Drilling. MC3 sample is being drilled with a dremmel tool by DAM in the ancient DNA laboratory.

Photo 4: Detail of drilling

Photo 5: Bone sampling. A portion of the MC3 has been drilled into a fine powder. This powder will be used in the subsequent DNA extraction.

Photo 6: Samples in early phase of ancient DNA extraction. Samples shown here are in the early phases of ancient DNA extraction. Most protocols require an initial demineralization step in EDTA pH 8.0, and need to rotate slowly in 56C. We seal the samples in a DNase/RNase free Sarstedt tube with EDTA, place them on an automatic rotator within an incubator that maintains a steady temperature.

Photo 7: Samples undergoing Phenol-chloroform DNA extraction. These samples are being extracted using a phenol-chloroform method. The samples have just had chloroform added to them.

Photo 8: Extraction outcome. Shown here are the samples after having undergone a phenol-chloroform extraction. The actual sample is on the right and an extract control on the left.

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