The visitor center for the preserve is located at Fort Caroline National Memorial and the park operates a visitor contact station at the Kingsley Plantation site.

The facilities and grounds for Fort Caroline National Memorial Timucuan Preserve Visitor Center and Kingsley Plantation are open 9:00 a.m. to 5:00 p.m., daily.

The preserve headquarters is open 7:45 a.m. to 4:15 p.m., Monday through Friday; closed on all government holidays.

The Theodore Roosevelt Area is open from sunrise to sunset, however restroom facilities are only open between 9:00 a.m. and 4:30 p.m.

Cedar Point is open from sunrise to sunset.

The Ribault Monument and Spanish Pond, associated with Fort Caroline National Memorial, are open 9:00 a.m. to 4:45 p.m.

The Ribault Club, an interagency visitor center, is open Wednesday - Sunday, 9:00 a.m. to 5:00 p.m.

All National Park Service areas of the Timucuan Preserve are closed on Thanksgiving, December 25, and January 1.