Bicyclists may use all paved roadways within the park and should follow the same laws and regulations that apply to the safe operation of motor vehicles as required in local and state laws. In order to conform to state laws, bicycles should travel in single file and stay on the right side of the road. Bicyclists should be aware that these roads have vehicular traffic and practice good defensive cycling techniques. Regulations prohibit bicycling on the actual Historic Jamestowne site.
The Colonial Parkway, a 23-mile scenic three-lane highway, connects Yorktown Battlefield, Colonial Williamsburg and Historic Jamestowne. (These three sites are known as the Historic Triangle.) The Parkway starts at the Yorktown Battlefield Visitor Center parking, heads west following the York River to Williamsburg, turns south to follow the James River, and ends up in the Historic Jamestowne Visitor Center parking lot.
The National Park Service maintains and patrols the Parkway. Pull-offs of historical significance along the Parkway provides scenic views and historical information. In keeping with the natural beauty and the Colonial atmosphere of the route, the three-lane roadway is not marked with white or yellow dividing lines. Composed of a cobble aggregate pavement, the highway simulates an early Colonial Virginia roadway and can present a rough, bumpy ride for bicyclists. The center lane of this three-lane roadway is for passing only; signs indicate no passing zones.