Beginning in the summer of 2003, a survey of Antietam's fish will be conducted in cooperation with Frostburg State University. The survey goals include determining the distributions and densities of fish in the park, establishing a baseline for continued health and population monitoring, obtaining an idea of the health of the streams based on fish populations and collecting preliminary water chemistry and physical habitat data. The Park Service plans on using the information collected as part of a larger effort to help develop a management plan for aquatic resources in the region. Up until now, only the following species have been documented in the park:

Brown Bullhead

American Eel

Central Stoneroller

White Sucker

Checkered Sculpin

Spotfin Shiner

Carp Greenside

Darter Tessellated

Darter Northern

Hogsucker Green

Sunfish Bluegill

Common Shiner

Smallmouth Bass

Largemouth Bass

Redhorse Sucker

Rosyface Shiner

Rainbow Trout

Yellow Perch

Bluntnose Minnow

Blacknose Dace

Longnose Dace

Brown Trout

Brook Trout

Creek Chub