The National Park Service and the Minnesota Department of Natural Resources (DNR) surveyed the 72 miles of Missisippi River and 4 miles of Minnesota River that constitute the Mississippi National River and Recreation Area. The Minnesota DNR began this important work with the Statewide Mussel Survey, during the 1999 field season. The statewide survey continued during the 2000 field season and in the 2001 season with the involvement of the National Park Service's Inventory and Monitoring Program.
During the 2000 field season, the DNR was able to sample a total of 60 sites along the metropolitan Mississippi River corridor. In 2001, 108 sites were sampled. A total of 13,589 live mussels were collected over the two survey years. Some species were found in one year and not in the other. Forty-two of Minnesota's 49 species were found in this stretch of river; 22 of those were live.