San Cayetano de Calabasas: About halfway between Nogales and Tumacácori, one mile east of highway I-19, are the adobe remnants of Calabasas, a site first occupied about 1756 as a Spanish mission visita (a mission station, usually a small Indian village but without a resident priest). Father Pedro Font held mass here on October 17, 1775, as the Anza expedition moved toward Tubac. The site is part of Tumacácori National Historical Park.

San José de Tumacácori: Located on highway I-19 about 18 miles north of Nogales, Tumacácori was first listed in 1691 as an outlying visita by the famous Jesuit missionary Father Eusebio Francisco Kino. By 1701, the village was a visita of the mission at Guevavi, and in 1771, under the Franciscans, the village with its primitive church was made the head mission of the district, and Guevavi was abandoned. Father Font spent several days at Tumacacori while Anza marshalled his forces at Tubac, and the mission contributed a small herd of cattle to the expedition. Construction of the present mission church was begun around 1802. Tumacácori is National Historical Park and includes the Calabasas and Guevavi sites. The visitor center is an NHL.

Presidio de San Ignacio de Tubac: Located four miles north of Tumacácori, the Presidio de San Ignacio de Tubac was founded in 1752 in response to a Pima revolt. The area had been a Pima village before becoming a mission farm. The fifty cavalrymen garrisoned at this remote outpost were to control the Pimas, protect the frontier from the Apaches and Seris, and further explore the Southwest. Juan Bautista de Anza II, second commander of the presidio, staged two overland expeditions to Alta California from this place. The ruins of his house can be viewed through an underground archeological exhibit at Tubac Presidio State Historical Park. About ten acres of the original site are within the state park and 23 acres are in private ownership. Thirteen acres of the private land are leased by the Center for Spanish Colonial Archeology. The Anza Trail runs through this property.