Big Cypress National Preserve is located in Southwest Florida between the cities of Miami and Naples. Interstate 75 (Alligator Alley) and U. S. Highway 41 (Tamiami Trail) are the main roads that traverse the site. Visitor facilities and most activities originate from the Tamiami Trail.
The Oasis Visitor Center is located along the Tamiami Trail, 50 miles west of Miami and 50 miles east of Naples. Preserve Headquarters is located near Naples and Everglades City, along U. S. 41, 5 miles east of the State Road 29 intersection.
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Click here to visit the Florida Highway Patrol's online state road map. The preserve is located on map section 6.
