1: Sample storage. Samples are stored
in DNase/RNase free Sarstedt tubes.
2: Post decontamination. Kennewick MC3
sample has been scrubbed with a 10% bleach solution and UV-irradiated
on all sides. Sample is ready for drilling.
3: Drilling. MC3 sample is being drilled
with a dremmel tool by DAM in the ancient DNA laboratory.
4: Detail of drilling
5: Bone sampling. A portion of the MC3
has been drilled into a fine powder. This powder will be used in the
subsequent DNA extraction.
6: Samples in early phase of ancient
DNA extraction. Samples shown here are in the early phases of ancient
DNA extraction. Most protocols require an initial demineralization step
in EDTA pH 8.0, and need to rotate slowly in 56C. We seal the samples
in a DNase/RNase free Sarstedt tube with EDTA, place them on an automatic
rotator within an incubator that maintains a steady temperature.
7: Samples undergoing Phenol-chloroform
DNA extraction. These samples are being extracted using a phenol-chloroform
method. The samples have just had chloroform added to them.
8: Extraction outcome. Shown here are
the samples after having undergone a phenol-chloroform extraction. The
actual sample is on the right and an extract control on the left.